TFTB1 [3916.120.15]細胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 小鼠

運輸方式： 凍存運輸

ATCC Number： CRL-1759?

細胞形態(tài)： **樣

**類型： IgG1

TFTB1 [3916.120.15]細胞Designations: TFTB1 [3916.120.15]

Depositors: R Fulton

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: TFTB1 [3916.120.15]細胞immunoglobulin; monoclonal antibody; against the B chain of the plant toxin ricin

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Comments: Animals were immunized with ricin B chain.

Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14).

The antibody does not cross-react with the ricin A chain.

TFTB1 [3916.120.15]細胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1321: Fulton RJ, et al. TFTB1 [3916.120.15]細胞Purification of ricin A1, A2, and B chains and characterization of their toxicity. J. Biol. Chem. 261: 5314-5319, 1986. PubMed: 3957927