器官來源： 眼
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-2502?
細胞形態(tài)： 上皮樣
運輸方式： 凍存運輸
細胞類型： 其他細胞類型
組織來源： retinal pigmented epithelium; retina
是否是腫瘤細胞： 0
物種來源： 人
數(shù)量： 大量
年限： 19 years
Designations: ARPE-19/HPV-16
ARPE-19/HPV-16細胞Depositors: JW Obringer
Biosafety Level: 2 [Cells contain Human Papilloma viral 16 (HPV16) sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: epithelial
Source: Organ: eye
Tissue: retinal pigmented epithelium; retina
Cell Type: human papillomavirus 16 (HPV-16) transfected
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche Transfection Reagents)
Antigen Expression: RPE-specific markers CRALBP and RPE-65 [34497]
DNA Profile (STR): Amelogenin: X, Y
CSF1PO: 11
D13S317: 11, 12
D16S539: 9, 11
D5S818: 13
D7S820: 9, 11
THO1: 6, 9.3
TPOX: 9, 11
ARPE-19/HPV-16細胞vWA: 16, 19
Cytogenetic Analysis: diploid
Age: 19 years
Gender: male
Comments: The ARPE/HPV-16 transformed cell line was derived from the ARPE-19 cell line (ATCC CRL-2302) by transfection with DH5-HPV-16.
ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident. [34497]
The cells express the RPE-specific markers CRALBP and RPE-65. [34497]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
ARPE-19/HPV-16細胞Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
Resuspend the cell pellet in fresh growth medium. ARPE-19/HPV-16細胞Add appropriate aliquots of the cell suspension to culture vessels.
Incubate cultures at 37C.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-2302
References: 34497: Dunn KC, et al. ARPE-19, A human retinal pigment epithelial cell line with differentiated properties. Exp. Eye Res. 62: 155-169, 1996. PubMed: 8698076
